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KMID : 0900919850090010036
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Korean journal of Animal Reproduction
1985 Volume.9 No. 1 p.36 ~ p.39
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Study on the Freezing of Bovine Embryos II. Bovine embryos survival after freezing and thawing
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Kim Choung-Ik
Yang Boo-Keun
Lee Sang-Young
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Abstract
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This experiment was carried out to investigate the morphology of bovine embryos thawed after deep freezing at -196^{circ}C and the development of frozen-thawed embryos after in vitro culture in Ham’s F-10 medium with 10% NBCS. The results obtained were summarized as follows: 1. The propotion of embryos which a, pp.ared mophologically normal was averaged 77.5% (79/102). 2. The morphologically normal rate of frozen-thawed blastocyst (78.6%) was higher than that of morula (76.7%), but there was no significant difference. 3. Normal development was observed in 20 of 68 embryos cultured for 24-72hr in medium and overall survival rate was 29.4%. 4. Survival rate fo blastocyst (33.3%) was higher than that of morula (25.7%).
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KEYWORD
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